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Resolve Chronic Inflammation and Achieve Healthy Ageing by Understanding Non-regenerative Repair

Animal research activities

Conduct of animal studies and protection of animals

The participants of RESOLVE conform to the current national and European legislation, regulations and ethical rules.

RESOLVE's principle handling with animal models is to limit experiments to the minimum required to generate meaningful data, to replace mouse models as a proof of principle as soon as available and to constantly refine experimental approaches for the benefit of the animals.

Normal repair

Organ: skin, liver
Used model: Balb/c, alphaMUPA

Inflammatory repair

Organs: skin, lung
Used model: Balb/c

Fibroproliferative repair

Organs: skin, lung, liver
Used models: Leptin -/-, C57BL/6, P19ARF-/- hepatocytes

Centenarian repair

Organs: skin
Used model: alphaMUPA

Skin wound healing

Organs: skin, limb muscles, heart, bone marrow
Used models: Balb/c, C57BL/6, p75NTR-/-

A standard wounding model in adult mice as well as a new wounding model, in which diabetes and ischemia are superimposed at the level of the lower extremities, are provided. Latter creates the typical clinical situation of ischemic diabetic ulcers.

Skin tissue are used for isolation of cytosolic RNA or stabilized for immunohistochemistry. Cells from each model are isolated and cultivated to analyse specifc processes relevant to re-epithelialization, granulation, tissue formation, neoangiogenesis and scar formation.

Pulmonary Wound Healing

RESOLVE performs animal model studies of pulmonary wound healing after inhalation of organic dusts or using bleomycin to induce lung lesions.

Bleomycin-induced lung fibrosis in C57BL6 mice

Liver transdifferentiation

With an especially useful repair model – taking advantage of a hepatocyte transformation model, which involves the epithelial to mesenchymal transition (EMT) of p19ARF-/- hepatocytes – RESOLVE analyses the influence of fibroproliferative repair on the epithelial differentiation repertoire.

Involvement

Francois Huaux (Université Catholique de Louvain) - workpackage-leader

Lars Peter Kamolz (Medizinische Universität Wien)
Vadim FraifeldRolf Ziesche (Medizinische Universität Wien)
Wolfgang Mikulits (Medizinische Universität Wien)
Jacek Dutkiewicz (Institute of Agricultural Medicine)
Marcin Golec
(Institute of Agricultural Medicine)
Vadim Fraifeld (Ben-Gurion University of the Negev)
Paolo Maddeddu (University of Bristol)